翁杰敏，男，1964年3 月17日出生，华一东师范大学生命医学研究所副所长，生物化学学科教授。主要研究方向便希敌溶当:表观遗传学，细胞核受体和肿瘤 。翁杰敏和妻子李纪文是华东师范来自大学生命科学学院生命医学研究所的双职工，两360百科人长期坚守教育科研岗位，自 1989 年赴美国留学至 2007 年，在生物医学领域取得了一定的成绩。回国后夫妇两人到我校工作 

• 中文名称 翁杰敏
• 国籍 中国
• 出生日期 1964年3 月17日
• 职业 教师
• 研究方向 表观遗传学

个人履历

　　1980.9-1984.7 武汉大学生物系 大学本科

翁杰敏和妻子李纪文

　　1984.9-1987.7 中科院上海细胞生物学研究所 硕士

　　1984.9-1994.3 The University of Vermont, USA 博士

　　1994.3-1997.7 National Institute of Health, USA 博士后

　　1997.8-2003.4 Assistant Professor, The Dep感京散和林船杂指句声artment of Molecular and Cellu滑跑客黄积都完lar Biology,

　　Baylor College of Medicine, Houston, Texas, USA

　　2003.4-2007.4 Associate Professor, The Department of Molecular and Cellular Biology,

　来自　Baylor College o渐无成活座输据没个省卫f Medicine, Houston, Texa未案依s, USA

　　2007.4- 华东师范大学生命科学学院，生物医学研究所教授

研究方向

　　翁杰敏实验室主要难族度委从事三个方面的研究工作。

　　第一是组蛋白化学修饰及其在基因表达中的作用。目前主要研究内容包括:(1)组蛋白甲基化是如何建立和去除的。(2)组蛋白化学修饰的信息是如何传递和解读的。(3)组蛋白化学修饰在维持干细360百科胞功能中如何起作用的。我们首次报告了在组蛋白H3 9号位赖氨酸甲基化甲状腺激素受体尔供校积界严调控基因表达中的作用。我们还研究了H3 9号位赖氨酸甲基化抑制基船宪景皇信限因表达的作用机制。另外，我们也探索了9号赖氨酸甲基化的信息是如何解读的。兰使秋温银格茶倒消我们发现并鉴定了一个新的9号赖氨酸甲基化识别蛋若香风里白，并证明了这一蛋白在异质染色体的形成或维持中起重要作用。这些研究不仅奠定了我们在组蛋白修饰方向的工作基础，也为我们最近开展的干细胞表观遗传学研究提供了良好的技术平台。

　　第二是细胞激素受体调控基因表达的分子宽财班笑机制。细胞核激素受体在将停就布美城胚胎发育、个体生长和发育、代谢聚、内分泌调控和癌变等多方面起着重要作用。一安阶由在含般而言，这些众多的生物学功费假失排能主要是通过其对靶基因表达调控而实现的。这方面主要的研究内容包括核激素受体共激活蛋白(coactivator)的结构功能和作用机制，共阻抑蛋白(corepressor)的作用机制和生物学功能。我们首次分离纯化了共阻抑距距逐神物买胶章密拉叶蛋白SMRT/N-CoR复合体，并阐明了HD去尼侵根论朝满AC3在介导 SMRT/N-CoR转录抑制功能中拉感香农关益罗流告世府的重要作用。另外我们最近发现了一个新的雄性激素受体共激活蛋白，这个共激活蛋白能特异促进雄性激素受体DNA结合能力。初步的研究表明这一蛋白在大部分前列腺癌样品中有过量表达，因此有望成为一个新的前列腺癌的分子标记物和治疗靶标。

　　第三个研究方向是人工诱导全能干细胞。掌重触西跑余新束凯轻细全能胚胎干细胞在再生医学和疾病治疗上具有广阔的前景，然而其应用却受到来源及伦理的制约。我们将研究直接用蛋白质转染细胞的方法诱导产生全能干细胞的可能性。

研究课题

　　a.组蛋白修饰和核小体改造对染色质结构和功能的调控

　　b. 组蛋白密码识别蛋白的鉴乐值京定和分子机制

　　c.组蛋白修饰的独特形式在干细胞更新、细胞分化和肿瘤发生等生理和病理过程中的重要作用

　　d.以雄性般尔激素受体作为模型，研究基因表达的基本机制

　　e. 雄性激素受体共激活蛋白(coacti来自vators)在前列腺癌中的作用。

　　f.共阻抑蛋白N-CoR/SMRT 复合体的作用机制和生理和病理功能

代表论文

　　(选自65篇SCI 文章)

　　1. Chen, H.B.,Wong, J., Jiang, K. and Bao, J.S. 1990. A new method for the synthesis of a structural gene. Nucleic Acid Research 18, 360百科871-878.

　　2.Wong, J., Liu, F., and B纸儿沿剂动北ateman. E. 1992入经很病能. Isolation of genomic DNA encoding transcription factor TFIID from Acanthamoeba castellan树练喜ii:: chara研方多富顶少cterization of the promoter. Nucleic Acids Res. 20, 4817-4826.

　　3. Radebaugh, C.A., Matthews, J. L., Geiss, G. K., Liu, F.,Wo作持伯都集送导ng, J., Bateman, E., Camier, S., 去须费路令冷利买Sentenac重章他, A. and Pa渐施福住级ule, M. R. 19免友少剂若群谈赵有94. TATA-box 火达材镇沿延者甚高binding protein (TBP) is 并氢立获报水维a constit的推讲理季致uent of the polymerase I-specific trans裂座元古协欢cription initia造三工额虽诗州象跟tion factor TIF-IB (SL1) bound to the 格秋受走接能月rRNA promoter and shows differential sensitivity to TBP-directed reagen达价ts in polymerase I, II, and III transcription factors. Mol. Cell. Biol. 14, 597-605.

　　4.Wong, J.and Bateman, E. 1994. TBP-DNA interactions in the minor groove discriminate between A:T and T:A base pairs. Nucleic Acids Res. 22, 1890-1896.

　　5. Ranjan, M.,Wong, J. and Shi, Y-B. 1994. Transcriptional repression of Xenopus TR gene is mediated by a thyroid hormone response element located near the start site. J. Biol. Chem. 269, 24699-24705.

　　6.Wong, J., Shi, Y-B. and Wolffe, A.P. 1995. A role of nucleosome assembly in the transcriptional regulation of Xenopus TRA gene by thyroid hormone receptor. Gene & Development. 9, 2696-2711.

　　7.Wong, J. and Shi, Y-B. 1995. Coordinated regulation of and transcriptional activation by Xenopus thyroid hormone and retinoid X receptors. J. Biol. Chem. 270, 18479-18483.

　　8.Wong, J., Shi, Y-B., and Wolffe, A.P. 1997. Determinants of chromatin disruption and transcriptional regulation instigated by the thyroid hormone receptor: hormone-regulated chromatin disruption is not sufficient for transcriptional activation. EMBO J. 16(11): 3158-3171.

　　9.Wong, J., Li, Q., Levi, B-Z., Shi, Y-B., and Wolffe, A.P. 1997. Structural and functional features of a specific nucleosome containing a recognition element for the thyroid hormone receptor. EMBO J. 16(23): 7130-7145.

　　10.Wong, J., Patterton, D., Imhof, A., Guschin, D., Shi, Y-B., and Wolffe AP. 1998. Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase. EMBO J. 17(2): 520-534.

　　12.Wong J., Liang, V.C., Sachs, L.M., and Shi, Y.B. 1998. Transcription from the thyroid hormone-dependent promoter of the Xenopus laevis thyroid hormone receptor betaA gene requires a novel upstream element and the initiator, but not a TATA Box. J Biol Chem 273:14186-93.

　　13. Xue, Y.,Wong, J., Moreno, G.T., Young, M.A., Cote, J., and Wang, W. 1998. NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities. Molecular Cell 2: 851-861.

　　14. Lanz, R. B., Mckenna, N.J., Onate, S.A., Albrecht, U.,Wong, J., Tsai, S.Y., Tsai, M-J. and O'Malley B.W. 1999. A steroid receptor coactivator, SRA, functions as an RNA and is present in an SRC-1 complex. Cell 97: 17-27.

　　15. Liu, Z.,Wong, J., Tsai, S.Y., Tsai, M-J and O'Malley B.W. 1999. Steroid receptor coactivator-1 (SRC-1) enhances ligand-dependent and receptor-dependent cell free transcription of chromatin. PNAS 96: 9485-9490.

　　16. Li, J., O'Malley B.W andWong J. 2000. p300 requires its HAT activity and SRC-1 interaction motif to facilitate TR activation in chromatin. Mol. Cell. Biol. 20: 2031-2042.

　　17. Li, J,. Wang, J; Wang, J; Nawaz, Z; Liu, J., Jun, Q., andWong, J. 2000. Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. EMBO J. 19(16): 4342-4350.

　　18. Wang, H., Huang, Z., Xia, L., Feng, Q., Erdjument-Bromage, H., Strahl, BD., Briggs, SD., Allis, CD.,Wong, J., Tempst, p and Zhang, Y. 2001. Methylation of Histone H4 at Arginine 3 Facilitates Transcriptional Activation by Nuclear Hormone Receptor. Science 293: 853-857.

　　19. Liu, Z.,Wong, J., Tsai, S.Y., Tsai, M-J and O'Malley B.W. 2001. Sequential recruitment of steroid receptor coactivator-1 (SRC-1) and p300 enhances progesterone receptor-dependent initiation and reinitiation of transcription from chromatin. PNAS 98: 12426-12431.

　　20. Huang, Z., Li, J. andWong, J. 2002. Androgen receptor possesses an intrinsic-hormone-independent trans-activation activity. Molecular Endocrinology. 16: 924-937.

　　21. Liu Z, Auboeuf D,Wong J, Chen JD, Tsai SY, Tsai MJ, O''Malley BW. 2002. Coactivator/corepressor ratios modulate PR-mediated transcription by the selective receptor modulator RU486. Proc Natl Acad Sci U S A. 99(12):7940-4.

　　22. Li, J; Lin, Q; Wade, P., Wang, W andWong, J. 2002. Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression. Genes & Development 16: 687-692.

　　24. Li, J., Lin, Q., Yoon, H-G., Huang, Z., Strahl, B.D., Allis, C.D., andWong, J.2002. The involvement of multiple histone methylation in transcriptional regulation by thyroid hormone receptor. Mol Cell Biol 22 (16): 5688-5697.

　　25.Wong J. 2002. Transcriptional regulation by thyroid hormone receptor in chromatin. Thyroid hormone receptors: Methods in Molecular Biology edited by Aria Baniahmad, p177-194.

　　26. Osborne CK, Bardou V, Hopp TA, Chamness GC, Hilsenbeck SG, Fuqua SA,Wong J, Schiff R, Allred DC and Clark GM. 2003. Overexpression of the Estrogen Receptor Coactivator AIB1 (SRC3) and the HER-2/neu Oncogene are Both Necessary for the Tamoxifen-Resistance in ER-Positive Primary Breast Cancers. J Natl Cancer Insti. 95(5):353-61.

　　27. Yoon HG, Chan DW, Huang ZQ, Li J, Fondell JD, Qin J andWong J. 2003. Purification and Functional Characterization of the Human N-CoR Complex: the Roles of HDAC3 and two WD-40 Repeat Subunits TBL1 and TBLR1. EMBO J 22(6):1336-1346.

　　28. Huang ZQ, Li J, Sachs LM, Cole PA andWong J. 2003. A Role for Cofactor-Cofactor and Cofactor-Histone Interaction in Targeting of CBP/p300, SWI/SNF and Mediator for transcription. EMBO J 22(9):2146-2155.

　　29. Yoon HG, Chan DW, Reynolds AB, Qin J andWongJ. 2003. Kaiso targets the N-CoR corepressor complex for DNA methylation mediated repression. Molecular Cell 12, 723-734.

　　30. Paul R. Thompson, Dongxia Wang, Ling Wang, Marcella Fulco, Natalia Pediconi, DianZheng Zhang, Woojin An, Qingyuan Ge, Robert G. Roeder,Wong J, Massimo Levrero, Vittorio Sartorelli, Robert J. Cotter, Philip A. Cole. 2004. Regulation of the p300 HAT Domain via a Novel Activation Loop. Nature Structural and Molecular Biology. 11(4): 308-15.

　　31. Wu RC, Qin J, Yi P,Wong J,Tsai SY, Tsai MJ, O''Malley BW. 2004. Selective Phosphorylations of the SRC-3/AIB1 Coactivator Integrate Genomic Reponses to Multiple Cellular Signaling Pathways. Molecular Cell.15(6):937-49.

　　32. Yoon HG, Choi Y, Cole PA andWong J. 2005 Reading and Function of a histone code involved in targeting corepressor complexes for repression. Mol Cell Biol;25(1):324-335.

　　33. Stewart MD, Li J andWong J. 2005 Relationship between H3 lysine 9 methylation, transcription repression and heterochromatin protein 1 recruitment. Mol Cell Biol;25(7):2525-38.

　　34. Zhang D, Yoon HG andWong J. 2005. JMJD2A is a novel N-CoR-interacting protein and is involved in repression of the human transcription factor achaete scute-like homologue 2 (ASCL2/Hash2). Mol Cell Biol;25(15):6404-14.

　　35. Burd CJ, Petre CE, Morey LM, Wang Y, Revelo MP, Haiman CA, Lu S, Fenoglio-Preiser CM, Li J, Knudsen ES,Wong Jand Knudsen KE. 2006 Cyclin D1b variant influences prostate cancer growth through aberrant androgen receptor regulation. Proc Natl Acad Sci U S A; 103:2190-5.

　　36. Yoon, H. G., andWong J. 2006. The corepressors silencing mediator of retinoid and thyroid hormone receptor and nuclear receptor corepressor are involved in agonist- and antagonist-regulated transcription by androgen receptor. Mol Endocrinol 20:1048-60.

　　37. Stewart D, Tomita A, Shi YB,Wong J. 2006. Chromatin immunoprecipitation for studying transcriptional regulation in Xenopus oocytes and tadpoles. Methods Mol Biol. 322:165-81.

　　38. Klose, R. Yamane JK, Bae Y, Zhang D, Erdjument-Bromage H, Tempst P,Wong Jand Zhang Y. 2006. The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36. Nature 442:312-316.

　　39. Li, J., Fu J, Toumazou C, Yoon HG, andWong J. 2006. A role of the amino-terminal (N) and carboxyl-terminal (C) interaction in binding of androgen receptor to chromatin. Mol Endocrinol 20:776-85.

　　40. Yamane, K., Toumazou C, Tsukada YI, Erdjument-Bromage H, Tempst P,Wong Jand Zhang Y. 2006. JHDM2A, a JmjC-Containing H3K9 Demethylase, Facilitates Transcription Activation by Androgen Receptor. Cell 125:483-95.

　　41. Stewart, M. D., J. Sommerville, andJ. Wong. 2006. Dynamic regulation of histone modifications in Xenopus oocytes through histone exchange. Mol Cell Biol 26:6890-901.

　　42. Fu, J., Yoon, HG., Qin, J. andWong J. 2007. Regulation of P-TEFb Elongation Complex Activity by CDK9 Acetylation. Mol Cell Biol. 27(13):4641-51.

　　荣誉

　　1997 National Research Service Award.

　　1996 Excellent Poster Award in Gordon Research Conference on "Hormone Action"

　　1997 The 1997 NIH Excellent Research Award.

　　1997 Excellent Poster Award in Gordon Research Conference on "Hormone Action"

　　2001 The 2001 Richard E. Weitzman Memorial Award from Endocrine Society

　　2004 2004 MCB Research Excellence Award.